145 research outputs found

    Adenylate effects on protein phosphorylation in the interenvelope lumen of pea chloroplasts

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    A 64-kilodalton (kDa) protein, situated in the lumen between the inner and outer envelopes of pea (Pisum sativum L.) chloroplasts (Soll and Bennett 1988, Eur. J. Biochem., 175, 301–307) is shown to undergo reversible phosphorylation in isolated mixed envelope vesicles. It is the most conspicuously labelled protein after incubation of envelopes with 33 nmol·1-1 [-32P]ATP whereas incubation with 50 mol·1-1 [-32P]ATP labels most prominently two outer envelope proteins (86 and 23 kDa). Half-maximum velocity for phosphorylation of the 64-kDa protein occurs with 200 nmol·1-1 ATP, and around 40 mol·1-1 ATP for phosphorylation of the 86- and 23-kDa proteins, indicating the operation of two distinct kinases. GGuanosine-, uridine-, cytidine 5-triphosphate and AMP are poor inhibitors of the labelling of the 64-kDa protein with [-32P]ATP. On the other hand, ADP has a potent influence on the extent of labelling (half-maximal inhibition at 1–5 mol·1-1). The ADP-dependent appearance of 32P in ATP indicates that ADP acts by reversal of kinase activity and not as a competitive inhibitor. However, the most rapid loss of 32P from pre-labelled 64-kDa protein occurs when envelope vesicles are incubated with ATP t1/2=15 s at 20 molsd1-1 ATP). This induced turnover of phosphate appears to be responsible for the rapid phosphoryl turnover seen in situ

    Many-body approach to proton emission and the role of spectroscopic factors

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    The process of proton emission from nuclei is studied by utilizing the two-potential approach of Gurvitz and Kalbermann in the context of the full many-body problem. A time-dependent approach is used for calculating the decay width. Starting from an initial many-body quasi-stationary state, we employ the Feshbach projection operator approach and reduce the formalism to an effective one-body problem. We show that the decay width can be expressed in terms of a one-body matrix element multiplied by a normalization factor. We demonstrate that the traditional interpretation of this normalization as the square root of a spectroscopic factor is only valid for one particular choice of projection operator. This causes no problem for the calculation of the decay width in a consistent microscopic approach, but it leads to ambiguities in the interpretation of experimental results. In particular, spectroscopic factors extracted from a comparison of the measured decay width with a calculated single-particle width may be affected.Comment: 17 pages, Revte

    Phase transition between synchronous and asynchronous updating algorithms

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    We update a one-dimensional chain of Ising spins of length LL with algorithms which are parameterized by the probability pp for a certain site to get updated in one time step. The result of the update event itself is determined by the energy change due to the local change in the configuration. In this way we interpolate between the Metropolis algorithm at zero temperature for pp of the order of 1/L and for large LL, and a synchronous deterministic updating procedure for p=1p=1. As function of pp we observe a phase transition between the stationary states to which the algorithm drives the system. These are non-absorbing stationary states with antiferromagnetic domains for p>pcp>p_c, and absorbing states with ferromagnetic domains for p≤pcp\leq p_c. This means that above this transition the stationary states have lost any remnants to the ferromagnetic Ising interaction. A measurement of the critical exponents shows that this transition belongs to the universality class of parity conservation.Comment: 5 pages, 3 figure

    Non-Perturbative QCD Treatment of High-Energy Hadron-Hadron Scattering

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    Total cross-sections and logarithmic slopes of the elastic scattering cross-sections for different hadronic processes are calculated in the framework of the model of the stochastic vacuum. The relevant parameters of this model, a correlation length and the gluon condensate, are determined from scattering data, and found to be in very good agreement with values coming from completely different sources of information. A parameter-free relation is given between total cross-sections and slope parameters, which is shown to be remarkably valid up to the highest energies for which data exist.Comment: 60 pages, Heidelberg preprin

    Contemporary percutaneous treatment of unprotected left main coronary stenoses: initial results from a multicenter registry analysis 1994-1996.

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    BACKGROUND: Coronary artery bypass surgery (CABG) has been considered the therapy of choice for patients with unprotected left main (ULMT) coronary stenoses. Selected single-center reports suggest that the results of percutaneous intervention may now approach those of CABG. METHODS AND RESULTS: To assess the results of percutaneous ULMT treatment from a wide variety of experienced interventional centers, we requested data on consecutive patients treated after January 1, 1994, from 25 centers. One hundred seven patients were identified who were treated either electively (n=91) or for acute myocardial infarction (n=16). Of patients treated electively, 25% were considered inoperable, and 27% were considered high risk for bypass surgery. Primary treatment included stents (50%), directional atherectomy (24%), and balloon angioplasty (20%). Follow-up was 98.8% complete at 15+/-8 months. Results varied considerably, depending on presentation and treatment. For patient

    Estimates of hadron azimuthal anisotropy from multiparton interactions in proton-proton collisions at sqrt(s) = 14 TeV

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    We estimate the amount of collective "elliptic flow" expected at mid-rapidity in proton-proton (p-p) collisions at the CERN Large Hadron Collider (LHC), assuming that any possible azimuthal anisotropy of the produced hadrons with respect to the plane of the reaction follows the same overlap-eccentricity and particle-density scalings as found in high-energy heavy ion collisions. Using a Glauber eikonal model, we compute the p-p eccentricities, transverse areas and particle-multiplicities for various phenomenological parametrisations of the proton spatial density. For realistic proton transverse profiles, we find integrated elliptic flow v2 parameters below 3% in p-p collisions at sqrt(s) = 14 TeV.Comment: 17 pages, 9 figures. Very minor mods. Version to appear in EPJ-

    S-Matrix Unitarity, Impact Parameter Profiles, Gluon Saturation and High-Energy Scattering

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    A model combining perturbative and non-perturbative QCD is developed to compute high-energy reactions of hadrons and photons and to investigate saturation effects that manifest the S-matrix unitarity. Following a functional integral approach, the S-matrix factorizes into light-cone wave functions and the universal amplitude for the scattering of two color-dipoles which are represented by Wegner-Wilson loops. In the framework of the non-perturbative stochastic vacuum model of QCD supplemented by perturbative gluon exchange, the loop-loop correlation is calculated and related to lattice QCD investigations. With a universal energy dependence motivated by the two-pomeron (soft + hard) picture that respects the unitarity condition in impact parameter space, a unified description of pp, pip, Kp, gamma* p, and gamma gamma reactions is achieved in good agreement with experimental data for cross sections, slope parameters, and structure functions. Impact parameter profiles for pp and longitudinal gamma* p reactions and the gluon distribution of the proton xG(x,Q^2,b) are calculated and found to saturate in accordance with S-matrix unitarity. The c.m. energies and Bjorken x at which saturation sets in are determined.Comment: 65 pages with 13 figures, Introduction, Sec. 3, and Conclusion extende
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